Method of forming a microarray from frozen tissue
AssigneeClinomics Biosciences, Inc.
InventorPatrick J. Muraca
Life of this patent
Estimating the term…
Abstract
A device particularly suited for constructing frozen tissue microarrays. The device comprises: a cooling chamber for receiving at least one frozen material and for maintaining the frozen material in a frozen condition; the cooling chamber moveable in an x and y direction relative to a horizontal surface; at least one coring needle comprising a cutting surface and a lumen for receiving a core of frozen material cut by the cutting surface; and at least one coring needle positioning element, for positioning the at least one coring needle over said frozen material for cutting said frozen material. Frozen tissue microarray blocks and methods of generating these are also provided.
Claims24
A method for forming a microarray block, comprising:
- (a) providing a donor block comprising a frozen tissue sample wherein said donor block is placed in a cooling chamber for receiving said frozen tissue sample and for maintaining said frozen tissue sample in a frozen condition;
- (b) obtaining a core of frozen tissue from said donor block;
- (c) providing a recipient block comprising a frozen embedding matrix;
- (d) generating a hole in said recipient block for receiving said core of frozen tissue;
- and
- (e) placing said core of tissue in said hole in said recipient block.
A method for forming a microarray block comprising:
- (a) providing a donor block comprising a frozen tissue sample wherein said donor block is placed in a cooling chamber for receiving said frozen tissue sample and for maintaining said frozen tissue sample in a frozen condition;
- (b) providing a recipient block comprising a frozen embedding matrix comprising at least one hole for receiving a core of frozen tissue;
- (c) obtaining a core of frozen tissue from said donor block;
- and
- (d) placing said core of tissue in said hole in said recipient block.
A method for forming a microarray block comprising:
- a) providing a device comprising:
- a fixed horizontal surface;
- a cooling chamber for receiving at least one frozen material and for maintaining said frozen material in a frozen condition;
- said cooling chamber moveable in an x- and y-direction relative to said fixed horizontal surface;
- at least one coring needle, said coring needle comprising a cutting surface and a lumen for receiving a core of frozen material cut by said cutting surface;
- and
- at least one coring needle positioning element, for positioning said at least one coring needle over said frozen material for cutting said frozen material;
- b) placing a frozen tissue sample in said cooling chamber;
- c) positioning said at least one coring needle over said tissue sample;
- d) obtaining a core of tissue sample from said frozen tissue sample using said at least one coring needle;
- e) placing a block of frozen embedding medium in said cooling chamber;
- f) positioning said at least one coring needle over said block of frozen embedding medium;
- g) coring a hole in said block of frozen embedding medium;
- and
- h) placing said core of tissue sample in said hole.
Dependencies are inferred from the claim text.