Method for non-homologous transformation of Yarrowia lipolytica
AssigneesInstitut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)
InventorsJean-Marc Nicaud, Claude Gaillardin, Georges Pignede
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Abstract
The invention concerns the integration of a gene of interest into the genome of a Yarrowia strain devoid of zeta sequences, by transforming said strain using a vector bearing zeta sequences.
Claims21
Method for integrating a gene of interest into the genome of a strain of Yarrowia, using a recombinant vector bearing an insert flanked by zeta sequences and comprising said gene of interest, characterized in that said recombinant vector is used to transform a strain of Yarrowia, the genome of which lacks zeta sequences.
Dependencies are inferred from the claim text.