C3 binding polypeptide of Streptococcus agalactiae group b Streptococcus
AssigneeRegents of the University of Minnesota
InventorsBeverly L. Smith, Patricia Ferrieri
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Abstract
This invention relates to the identification of a human complement C3 binding polypeptide and the nucleic acid which encodes the polypeptide from Streptococcus agalactiae. The polypeptide binds C3 and may be implicated in S. agalactiae adhesion and/or virulence. The polypeptide is conserved in mass in a variety of streptococcal isolates and is recognized by antibodies produced by humans exposed to or colonized with Group B Streptococcus.
Claims17
An isolated nucleic acid fragment that hybridizes to at least a portion of at least one of the nucleic acid fragments represented by SEQ ID NO:6 or SEQ ID NO:4 or their complementary strands under hybridization conditions of prehybridization for 1 hour at 62° C. in hybridization solution (5×SSC (1×SSC is 0.15 M NaCL, 0.015 M sodium citrate), 0.02% sodium dodecyl sulfate (SDS), 0.1% N-lauroylsarcosine, 1% Blocking Reagent) followed by two stringency washes with 2×SSC, 0.1% SDS for 5 minutes at room temperature and once with 0.5×SSC, 0.1% SDS for 15 minutes at 62° C., said isolated nucleic acid fragment encodes a polypeptide that binds human complement C3 protein.
An isolated nucleic acid having at least 50% nucleic acid identity to the nucleic acid fragments represented by SEQ ID NO:6 or SEQ ID NO:4, and which hybridizes under hybridization conditions of prehybridization for 1 hour at 62° C. in hybridization solution (5×SSC (1×SSC is 0.15 M NaCL, 0.015 M sodium citrate), 0.02% sodium dodecyl sulfate (SDS), 0. 1% N-lauroylsarcosine, 1% Blocking Reagent) followed by two stringency washes with 2×SSC, 0.1% SDS for 5 minutes at room temperature and once with 0.5×SSC, 0.1% SDS for 15 minutes at 62° C., to at least a portion of at least one of the nucleic acid fragments represented by SEQ ID NO:6 or SEQ ID NO:4 or their complementary strands, said nucleic acid encoding a polypeptide that binds human complement C3 protein.
An isolated polynucleotide encoding a polypeptide comprising the amino acids represented by SEQ ID NO:5.
An isolated nucleic acid fragment comprising SEQ ID NO:6 or SEQ ID NO:4 or their complementary strands.
An isolated nucleic acid fragment encoding a polypeptide having at least 50% amino acid identity to SEQ ID NO:5, said polypeptide binds to human complement C3 protein.
An isolated nucleic acid fragment encoding a polypeptide having at least 60% amino acid identity to SEQ ID NO:5, said polypeptide binds to human complement C3 protein.
An isolated nucleic acid fragment encoding a polypeptide having at least 70% amino acid identity to SEQ ID NO:5, said polypeptide binds to human complement C3 protein.
An isolated nucleic acid fragment encoding a polypeptide having at least 80% amino acid identity to SEQ ID NO:5, said polypeptide binds to human complement C3 protein.
An isolated nucleic acid fragment consisting essentially of at least 30 nucleotides of SEQ ID NO:4, wherein said nucleic acid fragment encodes a polypeptide that binds to human complement C3 protein.
Dependencies are inferred from the claim text.