Methods of using bioelastomers
AssigneeDuke University
InventorAshutosh Chilkoti
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Abstract
Bioelastomers are disclosed for use in methods of binding compounds including immunoassay methods, in biosensors and methods or regenerating biosensors, and in methods for targeting the delivery of a compound to a particular location within an animal subjects. In general, the bioelastomer is conjugated to a binding compound, which is in turn used to bind a compound of interest. For targeted compound delivery, the bioelastomer is conjugated to the compound to be delivered.
Claims27
An article having a regenerable patterned surface for binding compounds of interest from an aqueous solution, said article comprising:
- (a) a solid support having a plurality of separate and discrete hydrophobic surfaces formed thereon;
- and
- (b) a conjugate reversibly bonded to each of said separate and discrete hydrophobic surfaces, said conjugate comprising (i) a bioelastic compound and (ii) a binding compound, wherein said binding compound specifically binds a compound of interest, and wherein said bioelastic compound has a transition temperature below which said bioelastic compound is soluble in said solution, and above which said bioelastic compound is insoluble in said solution;
- so that said surfaces may be used to bind a compound of interest at a temperature above said transition temperature, and so that said conjugate can be removed from said hydrophobic surface for recycling of said article by lowering the temperature of said solid support below said transition temperature.
A method of recycling a regenerable article that has a patterned surface to which has been bound at least one compound of interest, said method comprising:
- (a) providing an article comprising a solid support having a plurality of separate and discrete hydrophobic surfaces formed thereon and a conjugate reversibly bonded to each of said separate and discrete hydrophobic surfaces, said conjugate comprising (i) a bioelastic compound and (ii) a binding compound, wherein said binding compound specifically binds a compound of interest, and wherein said bioelastic compound has a transition temperature below which said bioelastic compound is soluble in said solution, and above which said bioelastic compound is insoluble in said solution;
- and then
- (b) separating said conjugate from each of said separate and discrete hydrophobic surfaces by lowering the temperature of said article to below a transition temperature of the bioelastic compound;
- and
- (c) binding a second conjugate to each of said separate and discrete hydrophobic surfaces on said regenerated article, said second conjugate comprising a second bioelastic compound and a second binding compound, so that said article may be reused.
Dependencies are inferred from the claim text.