US 6,582,923 B2Granted

Method for analyzing polynucleotides

AssigneeVariagenics, Inc.

InventorsVincent P. Stanton, Jr., Jia Liu Wolfe, Tomohiko Kawate, Gregory L. Verdine

Life of this patent

Estimating the term…

The present invention relates to a method of synthesizing a polynucleotide with incorporation of a modified nucleotide.

Dependent claims

A method for synthesizing a polynucleotide, comprising mixing a a compound having the chemical structure:

wherein the “Base” is selected from the group consisting of adenine, cytosine, guanine or inosine, and thymine or uracil, with whichever three of the four nucleosides triphosphates, adenosine triphosphate, cytidine triphosphate, guanosine triphosphate and thymidine triphosphate, do not contain said base, in the presence of one or more polymerases.

Dependencies are inferred from the claim text.

C07H 19/10
Pyrimidine radicals: with the saccharide radical esterified by phosphoric or polyphosphoric acids
C07H 19/14
Heterocyclic radicals containing only nitrogen atoms as ring hetero atom: pyrrolo-pyrimidine radicals
C07H 19/20
Purine radicals: with the saccharide radical esterified by phosphoric or polyphosphoric acids
C07H 21/00
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate

From the US filing history. Foreign family members aren’t listed.

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