Method of extracting nucleic acids using particulate carrier
AssigneeToyo Boseki Kabushiki Kaisha
InventorsKatsuya Daimon, Shigeru Komai, Yutaka Takarada
Life of this patent
Estimating the term…
Abstract
The present invention provides a method of extracting and isolating nucleic acids from a material containing nucleic acids using a nucleic acid-binding particulate carrier. More specifically, the present invention provides a nucleic acid extraction method using a particulate carrier having a particle diameter of 0.5 to 15.0 μm, a pore diameter of 50 to 500 nm and a pore volume of 200 to 5000 mm3/g. According to the method of the invention, nucleic acids can be efficiently extracted from a biological material, in particular a material containing a large amount of contaminants, such as a clinical sample.
Claims17
A kit for extracting nucleic acids, comprising a nucleic acid-binding particulate carrier having a particle diameter of 0.5 to 15.0 μm, a pore diameter of 80 to 250 nm and a pore volume of 20 to 5000 mm3/g, a nucleic acid extraction solution for adsorbing nucleic acids to the particulate carrier, and a nucleic acid eluate for eluting the nucleic acids from a composite of the nucleic acids and the particulate carrier.
Dependencies are inferred from the claim text.