Method and a device for the evaluation of biopolymer fitness
AssigneeEvotec OAI AG
InventorsRudolf Rigler, Manfred Eigen, Karsten Henco, Ulo Mets, Jerker Widengren, Michael Stuke, Michael Brinkmeyer, Wolfgang Simm, Olaf Lehmann
Life of this patent
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Abstract
A method for identifying one or a small number of molecules, especially in a dilution of ≦1 μM, using laser excited FCS with measuring times ≦500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably ≦10−14 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.
Claims115
A device for detecting one or more molecules, molecular complexes or molecular fragments in a measuring volume by means of fluorescence spectroscopy which device comprises:
- a) a laser beam generation device for generating a laser beam with a first wavelength,
- b) a focusing device for focusing said laser beam onto the measuring volume wherein the laser beam is such highly focused within the measuring volume that in essence it exclusively covers the measuring volume,
- c) a detector device for detecting fluorescence radiation generated due to laser light excitation of one or more molecules, molecular complexes or molecular fragments in said measuring volume,
- d) a pinhole aperture arranged in an image plane within a beam path of fluorescence radiation confocally with respect to the measuring volume to limit the quantity of fluorescence radiation to be detected by the detector device,
- wherein the measuring volume is distanced from the focusing device by up to 1,000 μm.
A device for detecting one or more molecules, molecular complexes or molecular fragments in a measuring volume by means of fluorescence spectroscopy which device comprises:
- a) a laser beam generation device for generating a laser beam with a first wavelength,
- b) a focusing device for focusing said laser beam onto the measuring volume wherein the laser beam is such highly focused within the measuring volume that in essence it exclusively covers the measuring volume,
- c) a detector device for detecting fluorescence radiation generated due to laser light excitation of one or more molecules, molecular complexes or molecular fragments, said detector device being arranged in an image plane within a beam path of fluorescence radiation confocally with respect to the measuring volume to limit the quantity of fluorescence radiation to be detected,
- wherein the measuring volume is distanced from the focusing device by up to 1,000 micrometer.
Dependencies are inferred from the claim text.