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US 6,582,899 B1Granted
Methods for identifying agents that cause a lethal phenotype, and agents thereof
AssigneeDeltagen Proteomics, Inc.
InventorsCarl Alexander Kamb, Giordano Michael Caponigro
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Abstract
The present invention is directed to methods for performing negative selection assays leading to the identification of cytostatic or cytotoxic agents that cause a lethal phenotype. The invention is useful also for evaluation of conditional cytotoxicity and cell-specific cytotoxicity.
Claims30
A method for performing a selection for cytotoxic agents that cause a lethal phenotype, comprising the steps of:
- (a) introducing a genetic library encoding a plurality of putative cytotoxic agents into a population of target cells;
- (b) expressing said library in said population of target cells;
- (c) plating said population of target cells on a surface;
- (d) collecting a subpopulation of target cells that disattaches from said surface within a predetermined period of time;
- and
- (e) recovering a first sublibrary from said subpopulation,
- wherein said subpopulation of cells that disattaches from said surface is enriched for a lethal phenotype that correlates to the presence of said first sublibrary.
4 more elements
A method for obtaining cytotoxic agents that establish a lethal phenotype, comprising the steps of
- (a) providing a population of target cells with a genetic library encoding a plurality of putative cytotoxic agents;
- (b) expressing said library in said population of target cells;
- (c) collecting a subpopulation of cells that display a lethal phenotype;
- and
- (d) recovering a first sublibrary from said subpopulation;
- wherein said lethal phenotype correlates to the presence of said first sublibrary.
3 more elements
Dependencies are inferred from the claim text.