Natural fluorescent dye obtained from a marine invertebrate, compositions containing the said dye and their uses

A natural fluorescent dye obtained from an extract of a marine organism, said organism being a marine sea cucumber Holothuria scabra and said dye having the following characteristics:

• i. the fluorescent dye is decolorized by a reducing agent,
• ii. the fluorescent dye decolorized is not a synthetic compound,
• iii. the crude extract of the dye is yellowish green in color,
• iv. the purified dye is reddish brown colored powder when seen with the naked eye in the daylight,
• v. under tube light some hues of green are emitted by said dye,
• vi. the dye is amorphous in nature,
• vii. the dye is soluble in water,
• viii. the dye is insoluble in organic solvents,
• ix. the dye is negatively charged,
• x. the dye has a pH of 6.5,
• xi. the dye comprises a phenolic group,
• xii. the dye does not have a quinonoid ring,
• xiii. the dye does not have aromatic amine groups,
• xiv. the dye is non-proteinaceous in nature,
• xv. reducing sugar is absent from said dye,
• xvi. the dye can act as a biosurfactant,
• xvii. the dye has antimicrobial qualities and when antimicrobial assay is performed, shows zone of inhibition,
• xviii. pigment cum dye is a fluorescent dye and emits fluorescence when excited with different wavelengths of UV and visible spectral ranges on a spectrophotometer,
• xix. UV, visible spectroscopy is from 300 nm-700 nm and the peaks are marked at 379 nm and 439 nm wavelengths for said dye,
• xx. UV, visible spectroscopy from 250 nm-350 nm and the peaks are at 272 nm and 299 nm wavelengths,
• xxi. fluorescent spectroscopy for said dye in the UV and visible spectra, when excited with UV 270 nm wavelength the fluorescence is emitted in the 324 nm-380 nm range, said range being within the UVA wavelength range of ultraviolet rays of sunlight,
• xxii. at an excitation wavelength 450 nm in Fluorescent spectroscopy for said dye the fluorescence emission is at 500 nm-580 nm with maximum intensity,
• xxiii. at an excitation wavelength 540 nm in Fluorescent spectroscopy for said dye the fluorescence emission is at 500 nm-620 nm with maximum intensity,
• xxiv. with excitation wavelength 555 nm in Fluorescent spectroscopy for said dye the fluorescence emission is at 575 nm-620 nm with maximum intensity,
• xxv. physical checking of Whatman Filter no. 1 dipped with dye concentration 1:40000 dilution under UV transilluminator and Gel Documentation system with UV bulbs of 260 nm-280 nm range said dye emits a bluish green hue color of fluorescence,
• xxvi. the dye emits three different colored fluorescence at 3 different wavelengths of the UV and visible ranges of the fluorescent cubes of an epifluorescence microscope,
• xxvii. fluorescence blue color emission occurs in the 380 nm-400 nm range of UVA when excited under ultraviolet cube WU-330 nm-385 nm excitation range for said dye,
• xxviii. fluorescence yellow color emission occurs in the 500 nm-570 nm range when excited under WB cube of 450 nm-480 nm excitation range for said dye,
• xxix. fluorescence orange color emission occurs in the 570 nm-650 nm range when excited under WG cube of 510 nm-550 nm excitation range for said dye,
• xxx. the dye emits hues of grays under the ordinary transmitted light bulb of the epifluorescence microscope when seen under 10×objective,
• xxxi. the dye emits fluorescence colors at a dilution range of at least 1:40000,
• xxxii. the fluorescence of the extract persists after at least 1 year at room temperature,
• xxxiii. the fluorescence of the dye is photostable and is not deteriorated by long exposures to direct light, and
• xxxiv. the fluorescence of the dye does not change when frozen at 20° C.