Method for preparing a viral aerosol
AssigneeTransgene S.A.
InventorsClaude Sene, Didier Lamy
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Abstract
A method for preparing a viral aerosol from a dilute viral suspension prepared by dissolving a virus in an aqueous solution containing 6-12 g/l of a monovalent cation salt, or 50-100 g/l of a hexose, which is then nebulized with a gas pressure of 0.5-3.5 bars or an ultrasonic frequency of 2-5 MHz. The resulting aerosol composition is also disclosed.
Claims5
A method of increasing the transport of Cl— ions in a lung of a Cystic Fibrosis (CF) patient, the method comprising administering to the patient through the use of an aerosol a therapeutically effective amount of a viral aerosol comprising replication defective adenoviral particles encoding a functional CFTR protein, wherein a sufficient quantity of CFTR protein is produced to increase Cl— secretion in the lung, and wherein the viral aerosol is obtained by:
- (a) preparing a dilute viral suspension containing 104 to 1013 pfu of the replication defective adenoviral particles in an aqueous solution, wherein the dilute viral suspension limits inactivation of the virus wherein the aqueous solution contains at least 6 to 12 g/l of a salt or a monovalent cation of 50-100 g/l of a hextrose, and
- (b) nebulizing the dilute viral suspension under conditions sufficient to produce a viral aerosol.
Dependencies are inferred from the claim text.