US 6,582,660 B1Granted

Control system for active programmable electronic microbiology system

AssigneeNanogen, Inc.

InventorsMichael J. Heller, Eugene Tu

Life of this patent

Estimating the term…

Loading patent…

Abstract

A system for performing molecular biological diagnosis, analysis and multi-step and multiplex reactions utilizes a self-addressable, self-assembling microelectronic system for actively carrying out controlled reactions in microscopic formats. These reactions include most molecular biological procedures, such as nucleic acid hybridization, antibody/antigen reaction, and clinical diagnostics. Multi-step combinatorial biopolymer synthesis may be performed. A controller interfaces with a user via input/output devices, preferably including a graphical display. Independent electronic control is achieved for the individual microlocations. In the preferred embodiment, the controller interfaces with a power supply and interface, the interface providing selective connection to the microlocations, polarity reversal, and optionally selective potential or current levels to individual electrodes. A system for performing sample preparation, hybridization and detection and data analysis integrates multiple steps within a combined system.

Claims23

Dependent claims

A controlled addressable, programmable electronic matrix device system having a plurality of individually addressable electrodes, comprising:

a power supply,
a user input system for receiving user input and generating an output corresponding thereto,
a controller adapted to receive the output from the input system and provide output including control signals utilized to generate independent electronic environments at the electrodes of the matrix device, the control signals including information relating to at least one parameter selected from the group consisting of:
polarity, the presence or absence of a connection between the power supply and an individual electrode, and the amount of current or potential supplied to the individual electrode,
an interface adapted to connect the output of the controller to the matrix device, and
an active, programmable electronic matrix device, the matrix device comprising:
a plurality of contact pads for contacting the interface and receiving the control signals,
a substrate, the substrate supporting the plurality of individually addressable electrodes, wherein the plurality of individual addressable electrodes have a permeation layer disposed thereon,
biological capture probes disposed attached to the permeation layer for interacting with the specific binding entities;
and an illumination source operatively positioned to provide radiation to the active matrix device.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application: 08/859,644Filed 20 May 1997 · continuation
Priority: 1 Nov 1993
Granted: 24 Jun 2003
Term ends: Not estimatedThe source records don’t support an estimate.

Classification70 codes

G11C 13/0019
Comprising cells based on organic memory material: comprising bio-molecules
B01J 19/0046
Chemical, physical or physico-chemical processes in general; Their relevant apparatus: sequential or parallel reactions, e.g. for the synthesis of…
B01J 19/0093
Chemical, physical or physico-chemical processes in general; Their relevant apparatus: microreactors, e.g. miniaturised or microfabricated reactors
B01L 3/502761
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…

Family

Application08/146,504Parent of this continuation-in-part · filed 1 Nov 1993
Application08/271,882Parent of this continuation-in-part · filed 7 Jul 1994
Application08/304,657Parent of this continuation · filed 9 Sep 1994
US 5,632,957 AParent of this continuation
Application08/304,657Parent of this continuation-in-part
US 6,017,696 AParent of this continuation-in-part
US 5,605,662 AParent of this continuation-in-part
US 6,582,660 B1This patent

From the US filing history. Foreign family members aren’t listed.