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AssigneeHer Majesty in Right of Canada as Represented by the Minister of Agriculture and Agri-Food Canada
InventorsF. William Collins, A. Bachir Sarr, David A. Fielder
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A process is described for the preparation f electrostatically-linked, aliphatic- or alicyclic-substituted anionic or cationic polysaccharide gels from readily available macroporous ionic polysaccharide chromatographic media, such as diethylaminoethyl (DEAE), quaternary aminoethyl (QAE) and sulfopropyl (SP) substituted polysaccharide gels. These novel gels are used for the isolation, recovery and purification of non-polar extractives using one or more extracting solvents from the group of lower alcohols, ketones, and water. The non-polar extractives may be alk(n)lyresorcinols, steroid, triterpenoid, cardiac glycosides and saponins, steryl ferulates and other phenolic acid conjugates, flavonoids, lipids, alcohol-soluble antimicrobials, prolamines or other alcohol-soluble proteolipid complexes.
A method of preparing an electrostatically-linked, aliphatic or alicyclic-substituted anionic or cationic polysaccharide gel from a macroporous ionic polysaccharide chromatographic matrix comprising the steps of:
An electrostatically linked, aliphatic- or alicyclic-substituted anionic or cationic polysaccharide gel comprising an ionic hydrophobic ligand selected from the group consisting of anionic and cationic detergents, electrostatically bonded to a macroporous ionic polysaccharide gel matrix, so that at least 70% of the available ionic sites of said ionic polysaccharide gel matrix are occupied by the ligand to form a modified hydrophobic phase component, wherein the ligand contains a strongly ionizable functional group of opposite charge to that of said ionic polysaccharide gel matrix.
Dependencies are inferred from the claim text.