Methods of reconstructed goat embryo transfer
AssigneeGenzyme Transgenics Corporation
InventorsYann Echelard, Esmail Behbodi, William Gavin, David Melican
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Abstract
The invention features methods of producing a mammal, e.g., a cloned or transgenic mammal. The method includes maintaining a reconstructed embryo in culture until the embryo is in the 2 to 8 cell stage of embryogenesis, transferring the embryo at the 2 to 8 cell stage into a recipient mammal, and allowing the embryo to develop into a mammal.
Claims73
A method of producing a cloned goat, comprising:
- maintaining a goat reconstructed embryo, wherein the reconstructed embryo is formed by introduction of an activated cell or cell nucleus into an enucleated oocyte, in culture until the embryo is in the 2 cell stage;
- transferring the goat embryo at the 2 cell stage into a recipient goat;
- and, allowing the reconstructed embryo to develop into a goat, to thereby produce a cloned goat.
A method of producing a cloned goat, comprising:
- maintaining an activated goat reconstructed embryo, wherein the reconstructed embryo is formed by introduction of a cell or cell nucleus into an enucleated oocyte, in culture until the embryo is in the 4 cell stage;
- transferring the goat embryo at the 4 cell stage into a recipient goat;
- and,
- allowing the reconstructed embryo to develop into a goat, to thereby produce a cloned goat.
A method of producing a cloned goat, comprising:
- maintaining an activated goat reconstructed embryo, wherein the reconstructed embryo is formed by introduction of a cell or cell nucleus into an enucleated oocyte, in culture until the embryo is in the 8 cell stage;
- transferring the goat embryo at the 8 cell stage into a recipient goat;
- and,
- allowing the reconstructed embryo to develop into a goat, to thereby produce a cloned goat.
A kit comprising a goat reconstructed embryo which is in the 2 cell stage.
A kit comprising a goat reconstructed embryo which is in the 4 cell stage.
A kit comprising a goat reconstructed embryo which is in the 8 cell stage.
Dependencies are inferred from the claim text.