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US 6,576,464 B2Granted
Methods for providing differentiated stem cells
AssigneeGeron Corporation
InventorsJoseph D. Gold, Jane S. Lebkowski
Life of this patent
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AssigneeGeron Corporation
InventorsJoseph D. Gold, Jane S. Lebkowski
Estimating the term…
This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in relatively undifferentiated cells in the population. Expression of the effector gene results in depletion of undifferentiated cells, or expression of a marker that can be used to remove them later. Suitable effector sequences encode a toxin, a protein that induces apoptosis, a cell-surface antigen, or an enzyme (such as thymidine kinase) that converts a prodrug into a substance that is lethal to the cell. The differentiated cell populations produced according to this disclosure are suitable for use in tissue regeneration, and non-therapeutic applications such as drug screening.
An isolated primate pluripotent stem (pPS) cell containing a nucleic acid molecule comprising the structure P-X, wherein:
A method of producing a population of differentiated cells, comprising
A method of depleting a cell population of undifferentiated stem cells, comprising genetically altering undifferentiated stem cells in the population so that they contain a nucleic acid molecule comprising the structure P-X, wherein X is a nucleic acid sequence encoding a product that is lethal to a cell in which it is expressed, and P is a transcriptional control element that causes X to be preferentially expressed in undifferentiated cells.
A method of depleting a cell population of undifferentiated stem cells, comprising:
Dependencies are inferred from the claim text.