Treating open skin lesions using extract of sea buckthorn

A method of preparing an extract comprising, in the absence of oxygen and metals,

• (A) preparing a mixture, in a non-metallic container, of dried sea buckthorn berries and about 2 to about 4 pbw per pbw of said berries of a vegetable oil selected from the group consisting of almond oil, borage oil, canola oil, grape seed oil, jojoba oil, olive oil, soybean oil, sunflower oil, avocado oil, carrot oil, coconut oil, wheat germ oil, and mixtures thereof;
• (B) purging said mixture of dissolved oxygen;
• (C) incubating said mixture at the temperature of about 35° C. to about 65° C. for a time sufficient to form an extract of liposoluble herbal components from said berries;
• (D) separating said extract from said berries;
• and
• (E) repeating steps (A) to (D) at least once, each time using in step (A) the extract obtained from the previous steps.

A method of preparing an extract comprising

• (A) in a glass container placing a mixture of dried berries of sea buckthorn and about 2 to about 4 pbw per pbw of said berries of olive oil, and mixtures thereof;
• (B) sparging nitrogen through said mixture to remove dissolved oxygen;
• (C) incubating said mixture at the temperature of about 35° C. to about 65° C. for about 2 to about 4 days to form an extract of liposoluble herbal components from said berries;
• (D) separating said extract from said berries;
• (E) repeating steps (A) to (D) 1 to 3 times, each time using in step (A) the extract obtained from the previous steps;
• and
• (F) filtering the extract.