Methods and interferon deficient substrates for the propagation of viruses
AssigneeMount Sinai School of Medicine of New York University
InventorsPeter Palese, Adolfo García-Sastre, Robert O'Neil
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Abstract
The present invention relates, to novel methods and substrates for the propagation of viruses. The invention relates to IFN-deficient substrates and methods for propagating viruses in these unconventional substrates. In particular, the invention relates to methods of propagating viruses in immature embryonated eggs, preferably six- to nine-day-old chicken eggs. The methods of the invention are particularly attractive for growing viruses suitable for use in vaccine and pharmaceutical formulations.
Claims11
A method for vaccine production comprising:
- (a) propagating in an interferon deficient substrate an attenuated influenza virus having a mutation in the NS1 gene that diminishes or eliminates the ability of the NS1 gene product to antagonize the cellular interferon response;
- and
- (b) collecting progeny virus,
- wherein the interferon deficient substrate is the allantoic cavity of an immature embryonated egg six to nine days old or an interferon deficient cell line, with the proviso that said virus is not influenza C virus and the interferon deficient substrate is not Vero cells or STAT1(−) cell lines.
A method for vaccine production comprising:
- (a) propagating in embryonated eggs less than ten days old a recombinantly engineered attenuated influenza virus having a mutation in the NS1 gene that diminishes or eliminates the ability of the NS1 gene product to antagonize the cellular interferon response;
- and
- (b) collecting progeny virus,
- wherein the virus is grown to sufficient quantities and under conditions that are free from contamination, such that the progeny virus is suitable for formulation into a vaccine, with the proviso that said virus is not influenza C virus.
A method for vaccine production comprising:
- (a) propagating influenza virus in the allantoic cavity of an immature embryonated chick egg six days old;
- and
- (b) collecting progeny virus,
- wherein the virus is grown to sufficient quantities and under conditions that are free from contamination, such that the progeny virus is suitable for formulation into a vaccine, wherein the influenza virus is influenza A or B.
Dependencies are inferred from the claim text.