Process for mycelial culture using grain
AssigneesJae-Mahn Song, C.A. Biotech Co., Ltd.
InventorsJae-Mahn Song, Se-Youn Han, Yun-Sun Na
Life of this patent
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Abstract
Disclosed are a method for culturing mushroom mycelia using grains, a culture product, and use of the culture product. Edible or medicinal mushroom mycelia are inoculated and cultured in solid media made of grains. Induction of the cultured mushroom mycelia to undergo autolysis produces autolysates rich in antitumorigenic and other medicinally useful materials. The squeezing of the autolysates produces a liquid filtrate, leaving a paste. The filtrate is concentrated for use in foods or medicines. The paste is processed into a nutrient-rich gruel or other foods.
Claims7
A method for preparing products from cultured mushroom mycelia, comprising the steps of:
- preparing a solid medium by boiling or soaking a grain in 2 or 3 equivalents of water, placing the grain in a culture vessel, autoclaving and cooling the grain;
- inoculating a mushroom mycelium in an incubator at 20 to 30° C. for 15 to 30 days to root the mushroom mycelium into the solid medium;
- inducing autolysis of the mushroom mycelium grown into the grain substrate medium by treating the culture vessel at 45 to 55° C. for 24 to 48 hours;
- squeezing the autolysate to produce a liquid and concentrating the liquid;
- and
- drying the paste to a powder, wherein the paste is the solid extract from which the liquid is removed.
A method for preparing a gruel from a mixture of a grain and autolysed mushroom mycelium, comprising the steps of:
- preparing a solid medium by boiling or soaking a grain in 2 to 3 weights of water, placing the grain in a culture vessel, autoclaving and cooling the grain;
- inoculating a mushroom mycelium into the solid medium and culturing the mushroom mycelium in an incubator at 20 to 30° C. for 15 to 30 days to root the mushroom mycelium into the solid medium;
- mixing the mushroom culture, the grain, and water in proportions of 4:4:1;
- incubating the mixture at 60° C. for 16 hours to induce the autolysis of the mushroom mycelium and the hydrolysis of the grain, simultaneously;
- and
- heating the autolysates and hydrolysates at 80° C. for 30 mm to give a gruel.
A method for preparing a powder from a dried mixture of a grain and autolysed mushroom mycelium, comprising the steps of:
- preparing a solid medium by boiling or soaking a grain in 2 to 3 weights of water, placing the grain in a culture vessel, autoclaving and cooling the grain;
- inoculating a mushroom mycelium into the solid medium and culturing the mushroom mycelium in an incubator at 20 to 30° C. for 15 to 30 days to root the mushroom mycelium into the solid medium;
- mixing the mushroom culture, the grain, and water in proportions of 4:4:1;
- incubating the mixture at 60° C. for 16 hours to induce the autolysis of the mushroom mycelium and the hydrolysis of the grain, simultaneously;
- heating the autolysates and hydrolysates at 80° C. for 30 mm to give a gruel;
- and
- drying the gruel with hot air to produce a gruel powder.
Dependencies are inferred from the claim text.