Flavivirus and alphavirus recombinant antigen libraries
AssigneeMaxygen, Inc.
InventorsJuha Punnonen, Steven H. Bass, Robert Gerald Whalen, Russell Howard, Willem P. C. Stemmer
Life of this patent
Estimating the term…
Abstract
This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.
Claims51
A recombinant antigen library comprising recombinant nucleic acids that encode antigenic polypeptides, wherein at least one recombinant nucleic acid encodes at least one recombinant multivalent antigenic polypeptide comprising multiple non-contiguous subsequences of at least a first antigenic polypeptide of at least a first flavivirus or alphavirus and multiple non-contiguous subsequences of at least a second antigenic polypeptide of at least a second flavivirus or alphavirus, each subsequence being positioned relative to its position in the respective antigenic polypeptide, said at least one recombinant multivalent antigenic polypeptide inducing an immune response against each of the first and second antigenic polypeptides that is greater than the immune response induced by any one of the first and second antigenic polypeptides against any other of the first and second antigenic polypeptides.
A method of obtaining at least one polynucleotide that encodes at least one recombinant antigenic polypeptide that induces an immune response against different antigenic polypeptides, the method comprising:
- (1) recombining at least first and second nucleic acids, the first nucleic acid encoding at least a first antigenic polypeptide of a first flavivirus or alphavirus and the second nucleic acid encoding at least a second antigenic polypeptide of a second flavivirus or alphavivirus, wherein at least the first and second nucleic acids differ from each other in two or more nucleotides, to produce a library of recombinant nucleic acids that encode recombinant antigenic polypeptides;
- and
- (2) screening the library for at least one recombinant nucleic acid that encodes at least one recombinant antigenic polypeptide comprising multiple non-contiguous subsequences of at least the first antigenic polypeptide and multiple non-contiguous subsequences of at least the second antigenic polypeptide, each subsequence being positioned relative to its position in the respective antigenic polypeptide, which at least one recombinant antigenic polypeptide induces an immune response against each of the first and second antigenic polypeptides that is greater than the immune response induced by any one of the first and second antigenic polypeptides against any other of the first and second antigenic polypeptides.
A recombinant antigen library comprising recombinant nucleic acids that encode recombinant antigenic polypeptides, wherein the library comprises at least one recombinant nucleic acid that encodes at least one recombinant multivalent antigenic polypeptide comprising multiple non-contiguous subsequences of at least a first antigenic polypeptide of at least a first flavivirus or alphavirus, and multiple non-contiguous subsequences of at least a second antigenic polypeptide of at least a second flavivirus or alphavirus, each subsequence being positioned relative to its position in the respective antigenic polypeptide, said at least one recombinant multivalent antigenic polypeptide inducing production of neutralizing antibodies against at least the first and second antigenic polypeptides or the first and second flaviviruses or alphaviruses.
Dependencies are inferred from the claim text.