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AssigneeBayer CropScience AG
InventorsMichel Lebrun, Alain Sailland, Georges Freyssinet, Eric Degryse
Estimating the term…
The present invention relates to DNA molecules of plant origin encoding a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme wherein the a first EPSPS coding sequence that normally encodes a threonine residue of a mature EPSPS sequence is modified to encode isoleucine of the mature EPSPS sequence, and a second EPSPS coding sequence that normally encodes a proline residue of a mature EPSPS sequence is modified to encode serine of the mature EPSPS sequence, wherein the first and second residues are respectively located at relatively positions 102 and 106 of a mature EPSPS sequence encoded by said DNA molecule; and the production of a transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The mutated enzyme, which substantially maintains the catalytic activity of the wild-type enzyme, allows for increased tolerance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells.
A modified plant DNA molecule encoding a modified EPSPS enzyme wherein said enzyme comprises:
An isolated polynucleotide molecule having the sequence of SEQ ID NO:
A transformed plant cell comprising a vector comprising the following components, which are operably associated in the direction of transcription:
A transgenic plant comprising a vector comprising the following components, which are operably associated in the direction of transcription:
A method for selectively controlling plants which method comprises the steps of:
A plant transformed with a modified DNA molecule encoding a plant EPSPS enzyme having a first amino acid substitution from threonine to isoleucine at the threonine which corresponds to position 102 of the amino acid sequence of SEQ ID NO:
Dependencies are inferred from the claim text.