Gene for thermostable glucokinase, recombinant vector containing the same, transformant containing the recombinant vector and process for producing thermostable glucokinase using the transformant
AssigneeUnitika Ltd.
InventorsShido Kawase, Keisuke Kurosaka
Life of this patent
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Abstract
A gene encoding thermostable glucokinase, a recombinant vector comprising this gene, a transformant transformed with the recombinant vector, and a process for producing thermostable glucokinase with the use of the transformant.
Claims8
An isolated polynucleotide encoding a thermostable glucokinase, wherein said thermostable glucokinase comprises amino acids 1 to 317 of SEQ ID NO:1.
An isolated polynucleotide encoding a variant of a thermostable glucokinase, wherein said thermostable glucokinase comprises amino acids 1 to 317 of SEQ ID NO:1, and said variant has from 1 to 20 amino acid changes in SEQ ID NO:1 selected from the group consisting of deletions, substitutions or additions, and wherein said variant has thermostable glucokinase activity.
An isolated polynucleotide encoding a thermostable glucokinase, wherein said polynucleotide comprises nucleotides 1 to 954 of SEQ ID NO:2.
An isolated polynucleotide encoding a variant of a thermostable glucokinase, wherein said polynucleotide comprises nucleotides 1 to 954 of SEQ ID NO:2, wherein said polynucleotide has from 1 to 60 nucleotide changes selected from the group consisting of deletions, substitutions or additions, and wherein said polynucleotide encodes a polypeptide with thermostable glucokinase activity.
An isolated polynucleotide selected from the group consisting of:
- (a) an isolated polynucleotide that encodes a polypeptide comprising amino acids 1 to 317 of SEQ ID NO:1, and
- (b) an isolated polynucleotide comprising nucleotides 1 to 954 of SEQ ID NO:2.
Dependencies are inferred from the claim text.