Recombinant Zymomonas mobilis with improved xylose utilization
AssigneeMidwest Research Institute
InventorMin Zhang
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Abstract
A strain derived from Zymomonas mobilis ATCC31821 or its derivative capable of producing ethanol upon fermentation of a carbohydrate medium containing xylose to provide enhanced xylose utilization and enhanced ethanol process yield, the strain or its derivative comprising exogenous genes encoding xylose isornerase, xylulokinase, transaldolase and transketolase, the genes are fused to at least one promotor recognized by Zymomonas which regulates the expression of at least one of the genes.
Claims13
A Zymomonas mobilis strain ATCC31821/pZB5 capable of producing ethanol upon fermentation of a carbohydrate medium containing xylose to provide enhanced xylose utilization and enhanced ethanol process yield, said strain comprising exogenous genes encoding xylose isomerase, xylulokinase, transaldolase and transketolase, said genes are fused to at least one promotor recognized by Zymomonas which regulates the expression of at least one of said genes.
A process for production of ethanol from fermentable carbohydrate substrate medium comprising culturing in the medium without pH control, a strain of Zymomonas mobilis ATCC31821/pZB5 which is characterized by ethanol productivity at a temperature range of from about 25 to about 45° C. and utilizes about 99% of xylose to effect a fermentation reaction to provide improved yields of ethanol, and recovering the ethanol so produced.
A process for production of ethanol from a medium containing a fermentable carbohydrate substrate medium comprising culturing in the medium at a pH range of from about 4.5 to about 6.5 a strain of Zymomonas mobilis ATCC31821/pZB5 at a temperature range of from about 25 to about 45° C. and utilizes about 96% of xylose to effect a fermentation reaction to provide improved yields of ethanol, and recovering the ethanol produced.
A composition comprising a combination of a mixture of ATCC31821/pZB5 and ethanol.
Dependencies are inferred from the claim text.