# US 6,563,023 B2 · Identification and characterization of a CURLY phenotype (CUR) in plants

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- Number: US 6,563,023 B2
- Document ID: US6563023B2
- Status: Granted
- Kind: B2
- Application number: 09/874,926
- Filed: 2001-06-05
- Published: 2002-01-24
- Granted: 2003-05-13
- Earliest claimed: 2000-06-06
- Assignees: Agrinomics LLC
- Inventors: Alex Liu; Jill Van Winkle; Susan M. Bovee-Picciano; Stanley R. Bates; Helena V. Mathews; Ry Wagner
- Web page: https://patentgrep.com/patent/US6563023B2

## CPC

- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C07K 14/415: from plants
- C12N 15/8214: {Plastid transformation}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention is directed to a novel plant phenotype, designated CURLY (CUR), a nucleic acid sequence expressed in plants demonstrating the CUR phenotype and the corresponding amino acid sequence. Also provided are plant cells and plants that exhibit modified CUR expression.

## Claims

### Claim 1

An isolated polynucleotide comprising a nucleic acid sequence that encodes or is complementary to a sequence that encodes a CURLY polypeptide that has the amino acid sequence presented as SEQ ID NO:2.

### Claim 2 (depends on 1)

The polynucleotide of claim 1 comprising the nucleic acid sequence presented as SEQ ID NO:1, or the complement thereof.

### Claim 3 (depends on 1)

A plant transformation vector comprising an isolated polynucleotide of claim 1.

### Claim 4 (depends on 3)

A transgenic plant cell comprising the vector of claim 3.

### Claim 5 (depends on 3)

A method of producing a curled leaf phenotype in a plant, said method comprising introducing into progenitor cells of the plant a plant transformation vector according to claim 3 and growing the transformed progenitor cells to produce a transgenic plant, wherein said polynucleotide sequence is expressed and said transgenic plant exhibits a curled leaf phenotype.

### Claim 6 (depends on 5)

A plant obtained by a method of claim 5.

### Claim 7 (depends on 6)

A plant part obtained from a plant according to claim 6.