Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
AssigneeGenencor International, Inc.
InventorsBrian E. Jones, Wilhelmus A. H. Van Der Kleij, Piet Van Solingen, Walter Weyler, Frits Goedegebuur
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Abstract
A novel cellulase composition is provided which is produced by an Actinomycete. The cellulase has an approximate calculated molecular weight of 36 kD and has a pH optimum at 40° C. of 8 and at 60° C. of 7. Also provided is a DNA encoding said cellulase, a method for producing the cellulase and applications thereof.
Claims17
An expression vector comprising a regulatory polynucleotide sequence that is operably linked to a second polynucleotide sequence wherein the second polynucleotide sequence encodes a cellulase comprising an amino acid sequence provided in SEQ ID NO:1 or a derivative thereof having cellulolytic activity and at least 70% sequence identity to SEQ ID NO:1, wherein said regulatory polynucleotide sequence comprises a promoter derived from an Actinoplanes glucose isomerase gene and a signal sequence derived from a Streptomyces cellulase gene.
An expression vector comprising a regulatory polynucleotide sequence that is operably linked to a second polynucicotide sequence wherein the second polynucleotide sequence encodes a truncated cellulase having cellulolytic activity and comprising a contiguous amino acid sequence that is at least 90% identical to an amino acid sequence that corresponds to about position 31 and extends to about position 261 of SEQ ID NO:
- 1. wherein said regulatory polynucleotide sequence comprises a promoter derived from an Actinoplanes glucose isomerase gene and a signal sequence derived from a Streptomyces cellulase gene.
Dependencies are inferred from the claim text.