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US 6,558,899 B1Granted
Method for identifying new anti-picornaviral compounds
OwnerSimon K. Tsang, Diane M. Joseph-McCarthy +1inventors
InventorsSimon K. Tsang, Diane M. Joseph-McCarthy, James M. Hogle
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Abstract
The present invention is broadly directed to methods of screening viral-binding compounds. In particular, the present invention provides cell-free assays to rapidly screen libraries of compounds for viral-capsid binding activity. Such compounds are useful for anti-viral treatments.
Claims22
A method for screening compounds to determine anti-viral activity, comprising the steps of:
- incubating in vitro a mixture of two or more compounds with a viral site;
- allowing at least one of the two or more compounds to bind to the viral site;
- separating the at least one of the two or more viral site-bound compounds from an unbound compound;
- denaturing the viral site to release the compound from the viral site;
- detecting the at least one of the two or more released compounds;
- and
- testing the at least one of the two or more released compounds for anti-viral activity.
4 more elements
A method for screening compounds to determine anti-viral activity, comprising the steps of:
- incubating in vitro a mixture of two or more compounds with a viral site;
- allowing at least one of the two or more compounds to bind to the viral site;
- separating the at least one of the two or more viral site-bound compounds from an unbound compound;
- extracting the at least one of the two or more bound compounds into an organic phase,
- whereby the viral site is denatured and bound compound is released from the viral site;
- detecting the at least one of the two or more released compounds;
- and
- testing the at least one of the two or more released compounds for anti-viral activity.
5 more elements
Dependencies are inferred from the claim text.