α-glucuronidases of aspergillus, production thereof and their uses
AssigneeDanisco A/S
InventorsCharlotte Horsmans Poulsen, Masoud R. Zargahi, Ronald Peter de Vries, Jacob Visser
Life of this patent
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Abstract
Recombinantly produced α-glucuronidases which are useful in food manufacturing and as a feed additive to enhance the utilization of the feed components, and in other industrial applications such as pulp processing are provided. Genes coding for such enzymes can e.g. be isolated from Aspergillus sp. including A. tubigensis or A. niger.
Claims27
A method of enhancing the volume of a baked product made from a dough by at least 5%, the method comprising adding to the dough an effective amount of an alpha-glucuronidase that comprises at least one of:
- an alpha-glucuronidase naturally produced by an organism selected from the group consisting of Trichoderma reseei, Trichoderma viride, Aspergillus spp., Thermoascus aurantiacus, Agaricus bisporus and a Thermoanaerobacter sp, said alpha-glucuronidase exhibiting enzymatic activity at a pH in the range of 4 to 6.67 and having a molecular weight above 90 kDa.;
- an alpha-glucuronidase expressed by the phage lambda NCIMB 40801 and encoded by the coding sequence as shown in SEQ ID NO:
- 22;
- or
- an alpha-glucuronidase encoded by a DNA sequence that hybridizes to the coding sequence as shown in SEQ ID NO:
- 22 under the following conditions:
- pre-hybridization for 3 hours, hybridization under Southern blotting conditions at 65° C. and washing 2×20 minutes in 2×SSC, 0.5% SDS followed by 2×20 minutes in 0.2 SSC, 0.5% SDS,
- wherein the enhancement of the volume of the baked product made from a dough can be obtained without the addition of a further enzyme.
A method of improving the nutritional value of an animal feed component, the method comprising adding to the feed component a preparation comprising alpha-glucuronidase at an amount that, in the presence of enzymes of an animal digestive tract and in the absence of other enzymes, results in a reduction in residual dry matter of the feed component of at least 10%, wherein the alpha-glucuronidase comprises at least one of:
- an alpha-glucuronidase naturally produced by an organism selected from the group consisting of Trichoderma reseei, Trichoderma viride, Aspergillus spp., Thermoascus aurantiacus, Agaricus bisporus and a Thermoanaerobacter sp., said alpha-glucuronidase exhibiting enzymatic activity at a pH in the range of 4 to 6.67 and having molecular weight above 90 kDa;
- an alpha-glucuronidase expressed by the phage lambda NCIMB 40801 and encoded by the coding sequence as shown in SEQ ID NO:
- 22;
- or
- an alpha-glucuronidase encoded by a DNA sequence that hybridizes to the coding sequence as shown in SEQ ID NO:
- 22 under the following conditions:
- pre-hybridization for 3 hours, hybridization under Southern blotting conditions at 65° C. and washing 2×20 minutes in 2×SSC, 0.5% SDS followed by 2×20 minutes in 0.2 SSC, 0.5% SDS.
Dependencies are inferred from the claim text.