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US 6,555,732 B1Granted
Rac-like genes and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsJonathan P. Duvick, Yogesh Kumar Sharma
Life of this patent
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Abstract
The present invention provides methods and compositions relating to creating or enhancing disease resistance in plants. The invention provides isolated maize Rac nucleic acids and their encoded proteins that are involved in the altering the disease resistance pathway in plants, increasing transformation efficiency, inducing programmed cell death, and modulating the oxidative burst in a plant. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.
Claims29
An isolated polynucleotide selected from the group consisting of:
- (a) a polynucleotide consisting essentially of at least 25 contiguous bases of a polynucleotide selected from the group consisting of SEQ ID NOS:3, 5, 7, 9, 17, 19, 21, 23, 27, 29, 31, and 33;
- and
- (b) a polynucleotide consisting essentially of at least 50 contiguous bases of a polynucleotide selected from the group consisting of SEQ ID NOS:
- 1, 15, and 25.
1 more element
An isolated nucleic acid comprising a polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NOS:2, 4, 6, 8, 10, 16, 18, 20, 22, 24, 26, 28, 30, 32, and 34;
- (b) a polynucleotide selected from the group consisting of SEQ ID NOS:
- 1, 3, 5, 7, 9, 15, 17, 19, 21, 23, 25, 27, 29, 31, and 33;
- and
- (c) a polynucleotide that is complementary to the polynucleotide of (a) or (b).
2 more elements
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:1;
- (b) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:3;
- (c) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:5;
- (d) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:9;
- (e) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:15;
- (f) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:17;
- (g) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:19;
- (h) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:23;
- (i) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:25;
- (j) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:27;
- (k) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:29;
- (l) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:31;
- (m) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NO:33;
- and
- (n) a polynucleotide that is complementary to the polynucleotide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l), or (m).
12 more elements
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) the polynucleotide set forth in SEQ ID NO:1;
- (b) the polynucleotide set forth in SEQ ID NO:15;
- (c) the polynucleotide set forth in SEQ ID NO:25;
- (d) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:2;
- (e) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:16;
- (f) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:26;
- and
- (g) a polynucleotide complementary to the polynucleotide of (a), (b), (c), (d), (e), or (f).
5 more elements
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) the polynucleotide set forth in SEQ ID NO:3;
- (b) the polynucleotide set forth in SEQ ID NO:17;
- (c) the polynucleotide set forth in SEQ ID NO:27;
- (d) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:4;
- (e) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:18;
- (f) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:28;
- and
- (g) a polynucleotide complementary to the polynucleotide of (a), (b), (c), (d), (e), or (f).
5 more elements
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) the polynucleotide set forth in SEQ ID NO:5;
- (b) the polynucleotide set forth in SEQ ID NO:19;
- (c) the polynucleotide set forth in SEQ ID NO:29;
- (d) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:6;
- (e) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:20;
- (f) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:30;
- and
- (g) a polynucleotide complementary to the polynucleotide of (a), (b), (c), (d), (e), or (f).
5 more elements
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) the polynucleotide set forth in SEQ ID NO:7;
- (b) the polynucleotide set forth in SEQ ID NO:21;
- (c) the polynucleotide set forth in SEQ ID NO:31;
- (d) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:8;
- (e) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:22;
- (f) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:32;
- and
- (g) a polynucleotide complementary to the polynucleotide of (a), (b), (c), (d), (e), or (f).
5 more elements
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) the polynucleotide set forth in SEQ ID NO:9
- (b) the polynucleotide set forth in SEQ ID NO:23;
- (c) the polynucleotide set forth in SEQ ID NO:33;
- (d) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:10;
- (e) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:24;
- (f) a polynucleotide encoding the polypeptide set forth in SEQ ID NO:34;
- and
- (g) a polynucleotide complementary to the polynucleotide of (a), (b), (c), (d), (e), or (f).
5 more elements
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:1;
- (b) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:15;
- (c) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:25;
- and
- (d) a polynucleotide that is complementary to the polynucleotide of (a), (b), or (c).
2 more elements
An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:7;
- (b) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:21;
- (c) a polynucleotide encoding a polypeptide that enhances plant disease resistance, wherein said polynucleotide has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:31;
- and
- (d) a polynucleotide that is complementary to the polynucleotide of (a), (b), or (c).
2 more elements
Dependencies are inferred from the claim text.