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US 6,555,698 B1Granted
Chemiluminescent substrates for neuraminidase, assays for detection of neuraminidase and kits therefor
AssigneeTropix, Inc.
InventorsBrooks Edwards, Irena Bronstein, Rouh-Rong Juo
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Abstract
The present invention discloses chemiluminescent 1,2-dioxetane substrates capable of reacting with a neuraminidase to release optically detectable energy. These 1,2-dioxetanes have the general formula: wherein Z is and the variables are selected so as to induce decomposition of said dioxetane accompanied by chemiluminescence where Z is cleaved by neuraminidase present.
Claims21
An enzymatically cleavable chemiluminescent 1,2-dioxetane of formula I:
- which is capable of producing light energy when decomposed;
- wherein T is a substituted or unsubstituted polycycloalkyl group bonded to the 4-membered ring portion of said dioxetane by a Spiro linkage;
- wherein X is an aryl or heteroaryl moiety of 6-30 carbon atoms which induces chemiluminescent decomposition of said 1,2-dioxetane upon enzymatic cleavage of said moiety Z;
- wherein Z is an enzymatically cleavable group of formula II:
- wherein R1-R3 are each independently H or an alkyl (branched or straight chain) of 1-4 carbon atoms, and
- wherein R is an alkyl, aryl, aralkyl or cycloalkyl of 1-20 carbon atoms.
3 more elements
Dependencies are inferred from the claim text.