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US 6,555,674 B2Granted
JeT promoter
AssigneeNsGene A/S
InventorJens Tornøe
Life of this patent
Estimating the term…
Abstract
The JeT promoter is a recombinant promoter with transcriptional activity comparable to a number of strong mammalian promoters. The promoter consists of five key elements: (1) a TATA box; (2) a transcription initiation site (Inr); (3) a CAT consensus sequence in conjunction with (4) a CArG element and finally, (5) four Sp1 transcription binding sites (GGGCGG) arranged in two tandems.
Claims9
An isolated polynucleotide comprising SEQ. ID. NO:1.
An isolated polynucleotide comprising a functional derivative of SEQ ID NO:1 comprising the following elements and spacer sequences connected one to another in linear order:
- (a) a first element consisting of SEQ ID NO:6;
- (b) a first spacer sequence of any five deoxyribonucleotides;
- (c) a second element consisting of SEQ ID NO:6;
- (d) a second spacer sequence of any two deoxyribonucleotides;
- (e) a third element consisting of SEQ ID NO:4;
- (f) a third spacer sequence consisting of any 24 deoxyribonucleotides;
- (g) a fourth element consisting of SEQ ID NO:5;
- (h) a fourth spacer sequence consisting of any two deoxynucleotides;
- (i) a fifth element consisting of SEQ ID NO:6;
- (j) a fifth spacer sequence consisting of any five deoxyribonucleotides;
- (k) a sixth element consisting of SEQ ID NO:6;
- (l) a sixth spacer sequence consisting of any 14 deoxyribonucleotides;
- (m) a seventh element consisting of SEQ ID NO:2;
- (n) a seventh spacer sequence consisting of any 22 deoxyribonucleotides;
- (o) an eighth element consisting of SEQ ID NO:3.
12 more elements
Dependencies are inferred from the claim text.