Synthetic promoters

A promoter construct comprising:

• a) a synthetic core promoter functional in a plant cell, wherein said synthetic core promoter has a sequence comprising a TATA motif, a transcription start site, and a region between said TATA motif and said start site that is at least 64% GC-rich, and wherein said core promoter is a synthetic core promoter comprising the sequence set forth in SEQ ID NO:1;
• and
• b) at least two upstream activating regions, wherein at least one of said upstream activating regions is selected from the group consisting of Ubi-1 UAR and CaMV 35S UAR.

A promoter construct comprising:

• a) a synthetic core promoter functional in a plant cell, wherein said synthetic core promoter has a sequence comprising a TATA motif, a transcription start site, and a region between said TATA motif and said start site that is at least 64% GC-rich, and wherein said core promoter is a synthetic core promoter comprising the sequence set forth in SEQ ID NO:10;
• and
• b) at least two upstream activating regions, wherein at least one of said upstream activating regions is selected from the group consisting of Ubi-1 UAR and CaMV 35S UAR.

A promoter construct comprising the sequence set forth in SEQ ID NO:12.

A promoter construct comprising the sequence set forth in SEQ ID NO:15.

A promoter construct comprising the sequence set forth in SEQ ID NO:16.

A promoter construct comprising the sequence set forth in SEQ ID NO:17.

A promoter construct comprising the sequence set forth in SEQ ID NO:18.

A promoter construct comprising a sequence having at least 95% sequence identity to the portion of SEQ ID NO:12, which is not a CaMV 35S UAR sequence wherein control of transcription from said promoter is enhanced in comparison to transcription from a promoter consisting of the synthetic core promoter sequence set forth in SEQ ID NO:1.

A promoter construct comprising a sequence having at least 95% sequence identity to the portion of SEQ ID NO:15 which is not a Ubi-1 UAR sequence, wherein control of transcription from said promoter is enhanced in comparison to transcription from a promoter consisting of the synthetic core promoter sequence set forth in SEQ ID NO:1.

A promoter construct comprising a sequence having at least 95% sequence identity to the portion of SEQ ID NO:16 which is not a Ubi-1 UAR sequence, wherein control of transcription from said promoter is enhanced in comparison to transcription from a promoter consisting of the synthetic core promoter sequence set forth in SEQ ID NO:1.

A promoter construct comprising a sequence having at least 95% sequence identity to the portion of SEQ ID NO:17 which is not a Ubi-1 UAR sequence, wherein control of transcription from said promoter is enhanced in comparison to transcription from a promoter consisting of the synthetic core promoter sequence set forth in SEQ ID NO:1.

A promoter construct comprising a sequence having at least 95% sequence identity to the portion of SEQ ID NO:18 which is not a Ubi-1 UAR sequence, wherein control of transcription from said promoter is enhanced in comparison to transcription from a promoter consisting of the synthetic core promoter sequence set forth in SEQ ID NO:1.