US 6,555,659 B1Granted

Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5% w/w

OwnerJohn Stephen Ayers, Kay Patricia Coolbear +2inventors

InventorsJohn Stephen Ayers, Kay Patricia Coolbear, David Francis Elgar, Mark Pritchard

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Abstract

The present invention is related to a method for the purification of glycomacropeptide (GMP) with an amino acid composition containing no greater that 0.5% (w/w) phenylalanine, comprising the steps of contacting a GMP-containing feedstock with a first anion exchanger under conditions to adsorb the GMP, eluting the adsorbed GMP from the anion exchanger and removing impurities from the GMP-containing eluate by either: (i) contacting the GMP-containing eluate with a cation exchanger in conditions under which the impurities in the eluate are adsorbed onto the cation exchanger, or (ii) precipitating the impurities in GMP-containing eluate using conditions in which the GMP remains i solution, or (iii) contacting the GMP-containing eluate with a second anion exchanger in conditions under which the impurities in the eluate are adsorbed onto the anion exchanger, and recovering the GMP from whichever one or more of the steps (i), (ii) or (iii) was used.

Claims43

Dependent claims

A process for the purification of glycomacropeptide (GMP), comprising:

contacting a feedstock containing GMP with a first anion exchanger under conditions to adsorb said GMP;
obtaining an eluate containing GMP by eluting said adsorbed GMP from said anion exchanger with an eluent;
removing impurities from said eluate containing GMP, wherein said impurities comprise other whey proteins, by a technique selected from the group consisting of:
a) contacting said eluate containing GMP with a cation exchanger in conditions under which said impurities in said eluate are adsorbed onto said cation exchanger, and precipitating said impurities in said eluate containing GMP using conditions by means of which said GMP remains in solution,
b) precipitating said impurities in said eluate containing GMP using conditions by means of which said GMP remains in solution,
c) contacting said eluate containing GMP with a second anion exchanger in conditions under which said impurities in said eluate are adsorbed onto said anion exchanger, and
d) contacting said eluate containing GMP with a cation exchanger in conditions under which said impurities in said eluate are adsorbed onto said cation exchanger, and contacting said eluate containing GMP with a second anion exchanger in conditions under which said impurities in said eluate are adsorbed onto said anion exchanger;
and
recovering a purified GMP, wherein said purified GMP has an amino acid composition containing not greater than 0.5% (w/w) phenylalanine.

6 more elements

Dependencies are inferred from the claim text.