Compounds for diagnosis of tuberculosis and methods for their use
AssigneeCorixa Corporation
InventorsMark Raymond Alderson, Davin C. Dillon, Yasir A. W. Skeiky, Antonio Campos-Neto
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Abstract
Compounds and methods for diagnosing tuberculosis are disclosed. The compounds provided include polypeptides that contain at least one antigenic portion of one or more M. tuberculosis proteins, and DNA sequences encoding such polypeptides. Diagnostic kits containing such polypeptides or DNA sequences and a suitable detection reagent may be used for the detection of M. tuberculosis infection in patients and biological samples. Antibodies directed against such polypeptides are also provided.
Claims13
An isolated polypeptide comprising the amino acid sequence as set forth in SEQ ID NOs:126 or 142.
An isolated polypeptide from Mycobacterium tuberculosis encoded by a polynucleotide that hybridizes under moderately stringent conditions to a second polynucleotide that is complementary to the nucleotide sequence of SEQ ID NOs:125 or 140,
- wherein said moderately stringent conditions comprise prewashing in a solution of 5×SSC, 0.5% SDS, 1.0 mM EDTA (pH 8.0);
- hybridizing at 50-65° C. overnight in a solution comprising 5×SSC;
- and washing twice at 65° C. for 20 minutes with each of 2×, 0.5×, and 0.2×SSC containing 0.1% SDS.
Dependencies are inferred from the claim text.