Solution for fast visualization of protein
OwnerEwald M. Wondrakinventor
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Abstract
The present invention provides an improved solution for staining proteins in and/or on a solid matrix or support. Typically, a protein-containing gel or membrane may be washed in a hot solution of water for about five minutes, stained in a hot solution of COOMASSIE brilliant blue dye in dilute aqueous mineral acid for about five minutes, and then rinsed in water. The washing and/or staining steps may be performed by placing the gel in a wash and/or staining solution, respectively, heating the solution in which the gel is placed to boiling in a microwave oven, and incubating the gel in the solution for about five minutes. The entire procedure can be performed in a little over ten minutes, which represents an enormous time savings over existing methods.
Claims20
A solution comprising at least one brilliant blue dye as colloids in a dilute acid solution;
- wherein said dilute acid has a concentration equivalent to between about 1 mM and 100 mM, and said at least one brilliant blue dye has a concentration between 0.0005% (w/v) and 0.5% (w/v).
A solution comprising at least one brilliant blue dye as colloids in a dilute mineral acid solution;
- wherein said dilute mineral acid has a concentration equivalent to between about 5 mM and 100 mM, and said at least one brilliant blue dye has a concentration between 0.0005% (w/v) and 0.5% (w/v).
Dependencies are inferred from the claim text.