Process for production of mammalian cell lines
AssigneeTransgene S.A.
InventorsSerge Braun, Frederic Perraud
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Abstract
Described is a process for generating a mammalian cell line from primary mammalian cells, comprising the step of: a) pre-treating a culture of said primary mammalian cells or a suspension thereof with at least one glucocorticoid, b) optional step comprising obtaining a suspension of said pre-treated culture of step a), c) transferring into the pre-treated cells of the suspension of step a) or b) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells and d) culturing the transferred cells of step c). Furthermore, mammalian cell lines and cells obtainable by the described process are provided as well as pharmaceutical and diagnostic compositions containing such cells.
Claims21
A process for generating a mammalian cell line from primary mammalian cells, comprising:
- a) pre-treating a culture of primary mammalian cells or a suspension thereof with at least one glucocorticoid,
- b) if said culture of primary mammalian cells is not in the form of a suspension, obtaining a suspension of the pre-treated culture of step a),
- c) transferring into the pre-treated cells of the suspension at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells, and
- d) culturing the transferred cells of step c).
A process for generating a mammalian cell line from primary mammalian cells, comprising:
- a) obtaining a culture of said primary mammalian cells,
- b) pre-treating the culture of step a) with at least one glucocorticoid,
- c) obtaining a suspension of said pre-treated culture of step b),
- d) transferring into the pre-treated cells of the suspension of step c) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells and
- e) culturing the transferred cells of step d).
A process for generating a mammalian cell line from primary human muscle cells, comprising:
- a) obtaining a culture of said primary human muscle cells,
- b) obtaining a suspension of said cultured primary human muscle cells of step a),
- c) pre-treating the suspension of step b) with at least one glucocorticoid,
- d) transferring into the pre-treated cells of the suspension of step c) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells and
- e) culturing the transferred cells of step d).
Dependencies are inferred from the claim text.