Process of entrapping genetic materials in ultra-low size nanoparticles of inorganic compounds to form non-viral carriers

A process of entrapping genetic materials in nanoparticles of inorganic metal salts of size below 100 nm diameter to form non-viral carriers for delivery of genes comprising the steps of:

• (a) dissolving 0.01M to 1.0M of a surfactant or a mixture of surfactant and a cosurfactant in oil to obtain reverse micelles,
• (b) adding an aqueous solution of genetic material to the reverse micelles,
• (c) dividing the reverse micelles obtained in step (b) into two equal parts,
• (d) dissolving aqueous solution of 0.1 to 1.0M inorganic metal salts in one part of reverse micelles (step c) to obtain optically clear and transparent reverse micelles after dissolution,
• (e) adding aqueous solution of 0.1 to 1.0M precipitating agent in the second part of reverse micelles (step c) to obtain optically clear and transparent reverse micelles after dissolution,
• (f) maintaining the same molar ratio of water to surfactant in steps d and e,
• (g) mixing the reverse micelles of both steps (d) and (e) and stirring to form inorganic nanoparticles encapsulating added genetic material,
• (h) separating the nanoparticles from reverse micelles, and
• (i) dispersing the inorganic nanoparticles in water and dialyzing to remove free metal salts, surfactant and oil.