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US 6,555,375 B1Granted
Methods for somatic embryo formation and plant regeneration of Beta vulgaris
AssigneeAmerican Cyanamid Company
InventorAndrei E. Golovko
Life of this patent
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Abstract
The present invention relates to plant tissue culture methods, particularly for Beta vulgaris. Methods are provided for producing callus tissues, somatic embryos and plants. The methods find use in the field of agricultural biotechnology, particularly in the production of transgenic Beta vulgaris plants.
Claims30
A method for producing secondary somatic embryos of Beta vulgaris comprising:
- (a) exposing at least one somatic embryo to initiation medium;
- (b) isolating at least one embryogenic tissue mass that develops on said somatic embryo;
- (c) exposing said isolated embryogenic tissue mass to proliferation medium;
- (d) isolating an embryogenic outgrowth from said embryogenic tissue mass;
- and
- (e) exposing said isolated embryogenic outgrowth to regeneration medium to form a secondary somatic embryo.
3 more elements
A method for producing a Beta vulgaris plant comprising:
- (a) exposing at least one somatic embryo to initiation medium;
- (b) isolating at least one embryogenic tissue mass that develops on said somatic embryo;
- (c) exposing said isolated embryogenic tissue mass to proliferation medium;
- (d) isolating an embryogenic outgrowth from said embryogenic tissue
- (e) exposing said isolated embryogenic outgrowth to regeneration medium to form a secondary somatic embryo, and
- (f) regenerating said secondary somatic embryo into a plant.
3 more elements
Dependencies are inferred from the claim text.