Bifunctional selectable fusion genes

Abstract

The invention provides selectable fusion genes including a dominant positive selectable gene fused to and in reading frame with a negative selectable gene. The selectable fusion gene encodes a single bifunctional fusion protein which is capable of conferring a dominant positive selectable phenotype and a negative selectable phenotype on a cellular host. A dominant positive selectable phenotype is conferred, for example, by the hph gene for hygromycin B resistance (Hmr). A dominant negative selectable phenotype is conferred, for example, by the HSV-I TK gene for ganciclovir sensitivity (GCVs). A dominant positive selectable phenotype is also conferred by the neo gene for G-418 aminoglycoside antibiotic resistance (G-418r). A dominant negative selectable phenotype is also conferred by the CD gene for 5-fluorocytosine sensitivity (5-FCs). The present invention also provides recombinant expression vectors, such as retroviral vectors, which include selectable fusion genes, and cells transduced with the recombinant expression vectors. The bifunctional selectable fusion genes are expressed and regulated as a single genetic entity, permitting co-regulation and co-expression with a high degree of efficiency.

Dependencies are inferred from the claim text.