Flow injection flow cytometry system for on-line monitoring of biroreactors and method for monitoring
OwnerFriedrich Srienc, Rui Zhao +1inventors
InventorsFriedrich Srienc, Rui Zhao, Arvind Natarajan
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OwnerFriedrich Srienc, Rui Zhao +1inventors
InventorsFriedrich Srienc, Rui Zhao, Arvind Natarajan
Estimating the term…
For direct and on-line study of the physiological states of cell cultures, a robust flow injection system has been designed and interfaced with flow cytometry (FI-FCM). The core of the flow injection system includes a microchamber designed for sample processing. The design of the microchamber allows not only an accurate on-line dilution but also on-line cell fixation, staining, and washing. The flow injection part of the system was tested by monitoring the optical density of a growing E.coli culture on-line using a spectrophotometer. The entire growth curve, from lag phase to stationary phase, was obtained with frequent sampling. The system is thus particularly useful since it operates automatically without direct operator supervision for extended time periods.
A device for monitoring a biological factor comprising:
A method for direct monitoring a physiological state comprising the steps of:
Dependencies are inferred from the claim text.