Methods for microbial regulation
AssigneeUnisearch Limited
InventorsStaffan Kjelleberg, Peter David Steinberg, Peter Canisius De Nys, Ria Maximilien, Michael Manefield, Michael Givskov, Lone Gram
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Abstract
A method and microbial culture medium for inhibiting homoserine lactone and/or acylated homoserine lactone regulated processes in microorganisms using furanone compounds.
Claims42
A method of inhibiting an homoserine lactone and/or acylated homoserine lactone regulated process in a microorganism, the method comprising exposing a microorganism having an homoserine lactone and/or acylated homoserine lactone regulated process to an amount of a furanone compound having Formula 1 effective to inhibit the process but not to inhibit the growth of the microorganism,
- wherein:
- R1 is a hydrogen, hydroxyl, acetoxy, ester or ether group;
- R2 is Br or H;
- R3 and R4 are independently selected from the group consisting of a hydrogen atom and a halogen atom;
- and R5 is selected from the group consisting of C1, C3, C5 and C11 alkyl.
A method of inhibiting an homoserine lactone and/or acylated homoserine lactone regulated process in a microorganism, the method comprising exposing a microorganism having an homoserine lactone and/or acylated homoserine lactone regulated process to an amount of a furanone compound having Formula 2 effective to inhibit the process but not to inhibit the growth of the microorganism,
- wherein:
- R1, R2, R3 and R4 are each independently selected from the group consisting of a hydrogen, halogen, hydroxyl, methyl, alkyl, ether and ester group.
A method of inhibiting an homoserine lactone and/or acylated homoserine lactone regulated process in a microorganism, the method comprising exposing a microorganism having an homoserine lactone and/or acylated homoserine lactone regulated process to an amount of a furanone compound having Formula 1 effective to inhibit the process but not to inhibit the growth of the microorganism,
- wherein:
- compound 1 has R1=H, R2=Br, R3=Br, R4=Br, and R5=C3 alkyl;
- compound 2 has R1=H, R2=Br, R3=H, R4=Br, and R5=C3 alkyl;
- compound 3 has R1=OAc, R2=Br, R3=H, R4=Br, and R5=C3 alkyl;
- compound 4 has R1=OH, R2=Br, R3=H, R4=Br, and R5=C3 alkyl;
- compound 5 has R1=OAc, R2=Br, R3=H, R4=I, and R5=C3 alkyl;
- compound 8 has R1=H, R2=H, R3=Br, R4=Br, and R5=C3 alkyl;
- compound 10 has R1=OAc, R2=Br, R3=Br, R4=Br, and R5=C3 alkyl;
- compound 15 has R1=H, R2=Br, R3=Br, R4=Br, and R5=C1 alkyl;
- compound 16 has R1=H, R2=Br, R3=H, R4=Br, and R5=C1 alkyl;
- compound 17 has R1=H, R2=H, R3=Br, R4=Br, and R5=C1 alkyl;
- compound 12 has R1=H, R2=Br, R3=H, R4=Br, and R5=C5 alkyl;
- compound 13 has R1=H, R2=H, R3=Br, R4=Br, and R5=C5 alkyl;
- and
- compound 14 has R1=H, R2=H, R3=Br, R4=Br, and R5=C11 alkyl.
Dependencies are inferred from the claim text.