Methanotrophic carbon metabolism pathway genes and enzymes
AssigneeE. I. Du Pont de Nemours and Company
InventorsMattheos Koffas, Kelley C. Norton, James M. Odom, Rick W. Ye
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Abstract
Genes have been isolated from a Methylomonas sp encoding enzymes in the carbon flux pathway. The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.
Claims7
An isolated nucleic acid molecule encoding a Methylomonas sp phosphofructose kinase, selected from the group consisting of:
- (a) an isolated nucleic acid molecule encoding the amino acid sequence as set forth in SEQ ID NO:16;
- and (b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions:
- 0.1×SSC, 0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS;
- or an isolated nucleic acid molecule that is complementary to (a) or (b).
An isolated nucleic acid molecule comprising a first nucleotide sequence encoding a phosphofructose kinase of at least 437 amino acids having at least 80% identity when compared to a polypeptide having the sequence as set forth in SEQ ID NO:16,
- or a second nucleotide sequence comprising the complement of the first nucleotide sequence.
Dependencies are inferred from the claim text.