Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase

A method for dideoxy sequencing a nucleic acid molecule comprising:

• subjecting said nucleic acid molecule to a nucleic acid sequencing process with a three component DNA polymerase, wherein the three component DNA polymerase comprises a DNA polymerase component, a sliding clamp component, and a clamp loader component.

A kit for amplifying a nucleic acid molecule comprising a three component DNA polymerase comprising a DNA polymerase component, a sliding clamp component, and a clamp loader component and at least one deoxynucleoside triphosphate.

A kit for sequencing a nucleic acid molecule comprising a carrier means having in close confinement therein two or more container means, wherein a first container means contains a three component DNA polymerase comprising a DNA polymerase component, a sliding clamp component, and a clamp loader component and a second container means contains at least one dideoxynucleoside triphosphate.