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AssigneesStichting Instituut voor Dierhouderij en Diergezondheid, Bayer Aktiengesellschaft
InventorsDietmar Kretzdorn, Dirk Franciscus Marinus van de Wiel, Abraham Johannes de Smit, Robertus Jacobus Maria Moormann, Erik Kees Hamann
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The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1×105 to 5×106 cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or Em9 protein or fragments thereof in insect cell culture characterized by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 μg/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, α-units an/or β-units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 μl.
A method of producing an E2 subunit vaccine that includes a classical swine fever virus E2 protein without a transmembrane region, said method comprising:
The method of claim herein said adjuvant comprises a double water-in-oil emulsion.
A method of producing an E2 subunit vaccine that includes a classical swine fever virus E2 protein without a transmembrane region, said method comprising:
Dependencies are inferred from the claim text.