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AssigneeYamanouchi Pharmaceutical Co., Ltd.
InventorsMitsuyuki Matsumoto, Toru Sugimoto, Jun Takasaki, Masazumi Kamohara, Tetsu Saito, Masato Kobayashi
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This invention belongs to the genetic engineering field, and provides the novel G protein-coupled receptor family protein SREB2 expressed in the central nervous system, genes coding for the protein, screening methods using the protein and so on. As one of the methods for obtaining the G protein-coupled receptor protein of the present invention, RT-PCR is carried out using mRNA extracted from human or rat brain tissue or brain-derived cells as the template and using two primers interposing the entire portion or a part of the G protein-coupled receptor protein translation region, thereby obtaining cDNA corresponding to the G protein-coupled receptor protein or a part thereof, and the cDNA is integrated into an appropriate expression vector and expressed in a host cell.
An isolated polynucleotide encoding a G protein-coupled receptor protein comprising amino acids 1 to 370 of SEQ ID NO:
An expression vector comprising a polynucleotide encoding a G protein-coupled receptor comprising amino acids 1 to 370 of SEQ ID NO:
An isolated polynucleotide that is fully complementary to an isolated polynucleotide comprising a nucleotide sequence encoding a G protein-coupled receptor protein comprising amino acids 1 to 370 of SEQ ID NO:4.
An isolated polynucleotide encoding a polypeptide comprising amino acids 1 to 370 of SEQ ID NO:
An expression vector comprising a polynucleotide encoding a polypeptide comprising amino acids 1 to 370 of SEQ ID NO:
A method for producing a polypeptide comprising culturing the host cell of claim 25 under conditions sufficient for the production of said polypeptide and recovering said polypeptide from the culture.
An isolated polynucleotide that is fully complementary to an isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising amino acids 1 to 370 of SEQ ID NO:4.
An isolated polynucleotide comprising polynucleotides 1 to 1113 of SEQ ID NO:3.
Dependencies are inferred from the claim text.