NrdF from Staphylococcus aureus
AssigneeSmithKline Beecham Corporation
InventorsMichael Terence Black, Christopher Michael Traini
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Abstract
The invention provides nrdF polynucleotides encoding nrdF polypeptides from Staphylococcus aureus and methods for producing such polypeptides by recombinant techniques. The invention also provides polynucleotides having high identity to the nrdF polynucleotides. Also provided are preferred methods for utilizing these polynucleotides and polypeptides as diagnostic reagents and in diagnostic assays to screen for microbial infections in organisms and materials.
Claims18
An isolated polynucleotide segment, comprising a nucleic acid sequence, wherein the nucleic acid sequence encodes the same polypeptide, expressed by the nrdF gene encoded by a nucleotide sequence comprising SEQ ID NO:1 contained in Staphylococcus aureus contained in NCIMB Deposit No. 40771;
- wherein the nucleic acid sequence is not genomic DNA and wherein the nucleic acid sequence detects Staphylococcus aureus by hybridization.
An isolated polynucleotide segment comprising a nucleic acid sequence, wherein the nucleic acid sequence encodes a polypeptide comprising SEQ ID NO:2, wherein the nucleic acid sequence is not genomic DNA.
An isolated polynucleotide segment comprising a nucleic acid sequence, wherein the nucleic acid sequence encodes a polypeptide consisting of SEQ ID NO:2, wherein the nucleic acid sequence is not genomic DNA.
An isolated polynucleotide segment comprising a nucleic acid sequence, wherein the nucleic acid sequence comprises SEQ ID NO:1 and wherein the nucleic acid sequence is not genomic DNA.
Dependencies are inferred from the claim text.