Method to distinguish hematopoietic progenitor cells
AssigneeBecton, Dickinson and Company
InventorsJohanna Olweus, Fridtjof Lund-Johansen, Leon Wmm Terstappen
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Abstract
The expression of various cell adhesion molecules and growth factor receptor was examined on human progenitor cells (i.e., CD34+/CD38+). Certain changes in the expression if one or more of these molecules and receptors correlates with the progression of cell from non-lineage committed to commitment to a specific lineage. Using one or more markers for these molecules and/or receptors in combination with markers for CD34 and CD38 will enable one to identify and isolate each of the different progenitor cell populations.
Claims8
A method for identifying one or more populations of human progenitor cells, said populations comprising lymphoid, erythroid and myeloid progenitors, comprising the steps of labelling a sample of human cells containing said populations with a marker for CD34, a marker for CD38 and one or more markers for cell adhesion molecules and/or growth factor receptors and identifying said populations based upon the expression of each of the markers.
The method of claim i wherein said markers comprise fluorescently labelled monoclonal antibodies.
A method for isolating one or more populations of human progenitor cells, said populations comprising lymphoid, erythroid and myeloid progenitors, comprising the steps of labeling a sample of human cells containing said populations with a marker for CD34, a marker for CD38 and one or more markers for cell adhesion molecules and/or growth factor receptors and isolating said populations based upon the expression of each of the markers.
Dependencies are inferred from the claim text.