Methods for determining cell responses through EphB receptors
AssigneeVanderbilt University
InventorsThomas O. Daniel, Elke Stein
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Abstract
The present invention provides methods for screening an EphB receptor or an EphB receptor-binding ligand for the ability to promote a selected biological activity when in multimeric form. The invention also provides methods for initiating, promoting, directing, or inhibiting biological activities that involve EphB receptors and/or EphB receptor-binding ligands. The invention further provides compositions that can be used in the foregoing methods.
Claims8
A method for screening an EphB receptor-binding ligand for the ability to promote a selected biological activity when in multimeric form, comprising:
- a) contacting a multimeric EphB receptor-binding ligand with an EphB receptor-expressing cell under conditions whereby the ligand can bind the receptor;
- and
- b) detecting the selected biological activity in cells which have bound multimeric ligand as compared to the selected biological activity in cells which have not bound multimeric ligand, whereby a higher level of the selected biological activity in cells which have bound multimeric ligand compared to level of the selected biological activity of cells which have not bound multimeric ligand identifies an EphB receptor-binding ligand with the ability to promote the selected biological activity when in multimeric form.
A method for screening an EphB receptor for the ability to promote a selected biological activity when the receptor is in multimeric form, comprising:
- a) producing a multimeric EphB receptor on the surface of a cell which expresses an EphB receptor;
- and
- b) detecting the selected biological activity of cells with a multimeric EphB receptor as compared to the selected biological activity of cells without a multimeric EphB receptor, whereby a higher level of the selected biological activity of cells with a multimeric EphB receptor compared to the level of the selected biological activity of cells without a multimeric EphB receptor identifies an EphB receptor with the ability to promote the selected biological activity when in multimeric form.
Dependencies are inferred from the claim text.