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US 6,554,985 B2Granted
Methods and formulations for the separation of biological macromolecules
AssigneeCuraGen Corporation
InventorsMarie C. Ruiz-Martinez, Jan Berka, John W. Simpson
Life of this patent
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Abstract
This invention relates to methods and formulations for the separation of biological macromolecules according to their size using capillary electrophoresis with improved polyacrylamide matrixes under denaturing conditions.
Claims32
A replaceable matrix formulation comprising:
- (a) a linear polyacrylamide (LPA) solution, wherein the LPA concentration in said solution ranges between 1% to 3% (w/w);
- (b) at least one organic denaturant, wherein the organic denaturant is selected from the group consisting of:
- 2-pyrrolidinone, N′,N′-dimethylacetamide and N′,N′-dimethylformamide;
- (c) a buffer;
- and
- (d) 3 M to 8 M urea, wherein said formulation is capable of separating nucleic acids.
3 more elements
A replaceable matrix formulation comprising:
- (a) a linear polyacrylamide (LPA) solution, wherein the LPA concentration in said solution ranges from 1% to 3% (w/w);
- (b) at least one organic denaturant, wherein the organic denaturant is selected from the group consisting of:
- 2-pyrrolidinone, N′,N′-dimethylacetamide and N′,N′-dimethylformamide;
- (c) a buffer;
- and
- (d) 3 M to 8 M urea,
- wherein said formulation provides separation of nucleic acids sufficient for use in nucleic acid sequencing.
4 more elements
Dependencies are inferred from the claim text.