Compositions and methods for locating preferred integration sites within a plant genome

A method for locating preferred integration sites within a plant genome, said method comprising

• a) stably integrating into the genome of the plant a target site comprising a first nucleotide sequence operably linked to a promoter wherein said target site is flanked by a first recombination site and a second recombination site and wherein said first and said second recombination sites are non-identical;
• b) locating the preferred integration site by selecting the plant expressing said first nuclcotide sequence;
• c) transforming the plant expressing said first nucleotide sequence with a transfer cassette, said transfer cassette comprising a nucleotide sequence of interest flanked by the first and the second recombination sites;
• and,
• d) providing a recombinase that recognizes and implements recombination at the first and the second recombination sites,
• wherein said preferred integration site does not disrupt expression of an essential sequence and provides for adequate expression of said nucleotide sequence.

A method for locating preferred integration sites within a plant genome, said method comprising

• a) stably integrating into the genome of the plant a target site comprising a first nucleotide sequence operably linked to a promoter wherein the target site is flanked by a first recombination site and a second recombination site and wherein said first and said second recombination sites are non-identical;
• b) locating preferred integration sites by selecting the plant expressing said first nucleotide sequence and showing no significant negative impact on agronomic performance;
• c) transforming the plant expressing said first nucleotide sequence with a transfer cassette, said transfer cassette comprising a nucleotide sequence of interest flanked by the first and the second recombination sites;
• and,
• d) providing a recombinase that recognizes and implements recombination at the first and the second recombination sites.

A method for locating preferred integration sites within a genome of a plant cell, said method comprising

• a) stably integrating into the genome of said plant cell a target site comprising a first nucleotide sequence operably linked to a promoter wherein said target site is flanked by a first recombination site and a second recombination site and wherein said first and said second recombination sites are non-identical;
• b) locating preferred integration sites by selecting the plant cell expressing said first nucleotide sequence;
• c) transforming the plant cell expressing said first nucleotide sequence with a transfer cassette, said transfer cassette comprising a nucleotide sequence of interest flanked by the first and the second recombination sites;
• and,
• d) providing a recombinase that recognizes and implements recombination at the first and the second recombination sites,
• wherein said preferred integration site does not disrupt expression of an essential sequence and provides for adequate expression of said nucleotide sequence.