Process for the fermentative production of cephalosporin
AssigneeDsm N.V.
InventorsIlco Adrianus Lambertus Antonius Boogers, Emilius Johannes Albertus Xaverius Van De Sandt, Dick Schipper
Life of this patent
Estimating the term…
Abstract
A method for the recovery of a compound formula (I) from a complex mixture, comprising the steps of: (a) acidifying the complex mixture to a pH below 6.5 and maintaining the mixture below said pH at a temperature of between 50° C. and 130° C.; and/or (b) contacting the complex mixture with a carbon dioxide source; and (c) subjecting the mixture obtained after steps (a) and/or (b) to chromatography to obtain the compound of formula (I).
Claims17
A method for the recovery from a complex mixture of a compound of formula (I):
- R0 is hydrogen or C1-3 alkoxy;
- Y is CH2, oxygen, sulphur, or an oxidised form of sulphur;
- R1 is selected from the group consisting of
- hydrogen,
- hydroxy,
- halogen,
- C1-3 alkoxy,
- R2 is selected from the group consisting of adipyl, succinct, 2-(carboxyethylthio)acetyl, 3-(carboxyethylthio)propionyl, higher alkyl saturated dicarboxylic acids and higher alkyl unsaturated dicarboxylic acids,
- wherein said mixture comprises in addition to the compound of formula (I), 6-aminopenicillanic acid (6-APA) and optionally one or more additional N-substituted β-lactam compounds,
- comprising the steps of:
- (a) acidifying the complex mixture to a pH below 6.5 and maintaining the mixture below said pH at a temperature of between 50° C. and 130° C.;
- and/or
- (b) contacting the complex mixture with a carbon dioxide source;
- and
- (c) subjecting the mixture obtained after steps (a) and/or (b) to chromatography to obtain the compound of formula (I).
A method for the recovery from a complex mixture of a compound selected from the group consisting of adipyl-7-aminodesacetoxycephalosporanic acid (adipyl-7-ADCA), adipyl-7-aminodeacetylcephalosporanic acid (adipyl-7-ADAC) and adipyl-7-aminocephalosporanic acid (adipyl-7-ACA),
- wherein said mixture comprises in addition to said compound, 6-aminopenicillanic acid (6-APA) and optionally one or more additional N-substituted β-lactam compounds,
- comprising the steps of:
- (a) acidifying the complex mixture to a pH below 6.5 and maintaining the mixture below said pH at a temperature of between 50° C. and 130° C.;
- and/or
- (b) contacting the complex mixture with a carbon dioxide source;
- and
- (c) subjecting the mixture obtained after steps (a) and/or (b) to chromatography to obtain said compound.
Dependencies are inferred from the claim text.