Human MutT2 antibodies
AssigneeHuman Genome Sciences, Inc.
InventorsYing-Fei Wei, Ewen F. Kirkness
Life of this patent
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Abstract
A human hMutT2 polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for hydrolyzing and eliminating oxidized guanine nucleotides from the nucleotide pool to ensure correct DNA synthesis. Diagnostic assays are also disclosed which detect the presence of a mutated form of hMutT2 and over-expression of the hMutT2 protein.
Claims78
An isolated antibody or fragment thereof that specifically binds to a protein selected from the group consisting of:
- (a) a protein consisting of amino acid residues 1 to 219 of SEQ ID NO:2;
- (b) a protein consisting of a portion of SEQ ID NO:2, wherein said portion consists of at least 30 contiguous amino acid residues of SEQ ID NO:2 wherein said protein has human MutT2 activity;
- and
- (c) a protein consisting of a portion of SEQ ID) NO:2, wherein said portion consists of at least 50 contiguous amino acid residues of SEQ ID NO:2 wherein said protein has human MutT2 activity.
An isolated antibody or fragment thereof obtained from an animal that has been immunized with a protein selected from the group consisting of:
- (a) a protein comprising the amino acid sequence of amino acid residues 1 to 219 of SEQ ID NO:2;
- (b) a protein consisting of the amino acid sequence of at least 30 contiguous amino acid residues of SEQ ID NO:2 wherein said protein has human MutT2 activity;
- and
- (c) a protein consisting of the amino acid sequence of at least 50 contiguous amino acid residues of SEQ ID NO:2 wherein said protein has human MutT2 activity;
- wherein said antibody or fragment thereof specifically binds to said amino acid sequence.
An isolated monoclonal antibody or fragment thereof that specifically binds to a protein selected from the group consisting of:
- (a) a protein consisting of amino acid residues 1 to 219 of SEQ ID NO:2;
- (b) a protein consisting of a portion of SEQ ID NO:2, wherein said portion consists of at least 30 contiguous amino acid residues of SEQ ID NO:2 wherein said protein has human MutT2 activity;
- and
- (c) a protein consisting of a portion of SEQ ID NO:2, wherein said portion consists of at least 50 contiguous amino acid residues of SEQ ID NO:2 wherein said protein has human MutT2 activity.
An isolated antibody or fragment thereof that specifically binds to a protein selected from the group consisting of:
- (a) a protein consisting of the full-length polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882;
- (b) a protein consisting of a portion of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882, wherein said portion consists of at least 30 contiguous amino acid residues of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882 wherein said protein has human MutT2 activity;
- and
- (c) a protein consisting of a portion of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882, wherein said portion consists of at least 50 contiguous amino acid residues of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882 wherein said protein has human MutT2 activity.
An isolated antibody or fragment thereof obtained from an animal that has been immunized with a protein selected from the group consisting of:
- (a) a protein consisting of the amino acid sequence of the full-length polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882;
- (b) a protein consisting of the amino acid sequence of at least 30 contiguous amino acid residues of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882 wherein said protein has human MutT2 activity;
- and
- (c) a protein consisting of the amino acid sequence of at least 50 contiguous amino acid residues of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882 wherein said protein has human MutT2 activity;
- wherein said antibody or fragment thereof specifically binds to said amino acid sequence.
An isolated monoclonal antibody or fragment thereof that specifically binds to a protein selected from the group consisting of:
- (a) a protein consisting of the full-length polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882;
- (b) a protein consisting of a portion of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882, wherein said portion consists of at least 30 contiguous amino acid residues of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882 wherein said protein has human MutT2 activity;
- and
- (c) a protein consisting of a portion of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882, wherein said portion consists of at least 50 contiguous amino acid residues of the polypeptide encoded by the HE8AW20 cDNA contained in ATCC Deposit Number 75882 wherein said protein has human MutT2 activity.
An isolated antibody or fragment thereof that specifically binds a human MutT2 protein purified from a cell culture wherein the cells in said cell culture comprise a polynucleotide encoding amino acids 1 to 219 of SEQ ID NO:2 operably associated with a regulatory sequence that controls the expression of said polynucleotide.
Dependencies are inferred from the claim text.