Methods for enhancing binding interactions between members of specific binding pairs
AssigneeImmunivest Corporation
InventorsGalla Chandra Rao, Leon Terstappen, Paul Liberti
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Abstract
Disclosed is a method that improves the efficiency of reactions between specific binding pairs. By translating one member of the pair through a suspension of the second member, or across a surface to which the second member is immobilized, collision and binding frequencies are greatly increased. The resulting increased binding frequency allows for reduced incubation times and lower incubation temperatures. The enhanced collisions are advantageous for both molecular reactions, in which mixing is not always effective, and cellular reactions, in which the cells may be damaged by mixing.
Claims8
A method for enhancing binding interactions between members of a specific binding pair in a carrier medium, the first member being mobile throughout said carrier medium, and the second member being affixed to a magnetic particle, said particle also being mobile throughout said carrier medium, said method comprising performing a separation step immediately following introduction of one binding pair member into said carrier medium comprising the other binding pair member, said separation being caused by gravitational, electrical, or magnetic forces.
Dependencies are inferred from the claim text.